Analysis Note

ControlWestern Blot: Normal rat serum

Application

Research CategoryCell Structure

Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.

Immunogen

Purified rat plasma fibronectin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Antigen Affinity Purified

Quality

Routinely evaluated by Western Blot on normal rat serum.

Western Blot Analysis:
0.05 µg/mL - 1 µg/mL antibody detected fibronectin in 15 µL of normal rat serum.

Specificity

Other species have not been tested.

The antibody reacts with the 262 kDa fibronectin protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

262 kDa